Not Usually. There are certain vitamins that are heat sensitive and may be decreased in concentration due to the pasteurization process but to our knowledge, no cases of deficiency or hypovitaminosis have been attributed to proper pasteurization. There may be circumstances due to regional or farm-specific conditions that would dictate supplementation of vitamins, minerals or even added fat/protein. Always check with your local veterinarian if there are such suspicions and treat according to their instructions.